rabbit anti human ing4 polyclonal antibody  (Thermo Fisher)

Journal: Oncology Letters

Article Title: Association between the expression of inhibitor of growth family member 4 and the progression of clear cell renal carcinoma

doi: 10.3892/ol.2017.6450

Figure Lengend Snippet: ING4 mRNA is significantly decreased in CCRC tissues when compared with normal renal tissues. (A) A total of 40 paired tumor and adjacent normal renal tissues were analyzed for ING4 mRNA level by reverse transcription-quantitative polymerase chain reaction. β-actin was used as an internal control. (B) Analysis of relative mRNA level (compared with normal renal tissue) according to Fuhrman nuclear grade (24) (grade I, n=7; grade II, n=16; grade 3, n=13; grade 4, n=4), lymphatic metastasis (n0, n=29; n1, n=7; n2, n=4), tumor clinical stage (stage I, n=9; stage II, n=12; stage III, n=14; stage IV, n=5). ***P<0.001 by t-test. CCRC, clear cell renal carcinoma; ING4, inhibitor of growth family member 4.

Article Snippet: Briefly, 4-µm-thick sections were blocked with 5% bovine serum albumin in PBS prior to incubation overnight at 4°C with rabbit anti-human ING4 polyclonal antibody (1:100; cat no. 40-7700; Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques: Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Association between the expression of inhibitor of growth family member 4 and the progression of clear cell renal carcinoma

doi: 10.3892/ol.2017.6450

Figure Lengend Snippet: ING4 expression is significantly decreased in CCRC tissues when compared with normal renal tissues. (A) A total of 40 paired tumor and adjacent normal renal tissues were analyzed for ING4 expression using western blotting. Decreased ING4 expression was observed in CCRC tissues compared with adjacent normal renal tissues. β-actin was used as internal control. (B) Average ING4 expression in 40 paired tumor and normal renal tissues samples. (C) Analysis of the expression level of ING4 protein (compared with normal renal tissue) according to tumor nuclear grade (grade I, n=7; grade II, n=16; grade III, n=13; grade IV, n=4), lymphatic metastasis (n0, n=29; n1, n=7; n2, n=4), tumor clinical stage (stage I, n=9; stage II, n=12; stage III, n=14; stage IV n=5). ***P<0.001 by t-test. CCRC, clear cell renal carcinoma; ING4, inhibitor of growth family member 4; NT, normal tissue.

Article Snippet: Briefly, 4-µm-thick sections were blocked with 5% bovine serum albumin in PBS prior to incubation overnight at 4°C with rabbit anti-human ING4 polyclonal antibody (1:100; cat no. 40-7700; Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques: Expressing, Western Blot

Journal: Oncology Letters

Article Title: Association between the expression of inhibitor of growth family member 4 and the progression of clear cell renal carcinoma

doi: 10.3892/ol.2017.6450

Figure Lengend Snippet: ING4 protein expression detected using the EnVision system in (A) normal renal tubular epithelial cell nuclei, and in renal cell carcinoma; (B) nuclear grade I, cellular expression; (C) nuclear grade II, cell membrane and cytoplasmic expression; (D) nuclear grade III, cytoplasmic expression; (E) nuclear grade IV, cytoplasmic expression; (F) positive expression of cytoplasmic granules. Magnification of all images, ×400. ING4, inhibitor of growth family member 4.

Article Snippet: Briefly, 4-µm-thick sections were blocked with 5% bovine serum albumin in PBS prior to incubation overnight at 4°C with rabbit anti-human ING4 polyclonal antibody (1:100; cat no. 40-7700; Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Association between the expression of inhibitor of growth family member 4 and the progression of clear cell renal carcinoma

doi: 10.3892/ol.2017.6450

Figure Lengend Snippet: ING4 protein in different nuclear grade renal cell carcinoma tissues.

Article Snippet: Briefly, 4-µm-thick sections were blocked with 5% bovine serum albumin in PBS prior to incubation overnight at 4°C with rabbit anti-human ING4 polyclonal antibody (1:100; cat no. 40-7700; Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques:

Journal: Oncology Letters

Article Title: Association between the expression of inhibitor of growth family member 4 and the progression of clear cell renal carcinoma

doi: 10.3892/ol.2017.6450

Figure Lengend Snippet: ING4 protein expression in patients with clear cell renal carcinoma of the association between the clinical and pathological features.

Article Snippet: Briefly, 4-µm-thick sections were blocked with 5% bovine serum albumin in PBS prior to incubation overnight at 4°C with rabbit anti-human ING4 polyclonal antibody (1:100; cat no. 40-7700; Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques: Expressing